This secondary antibody is broad VHH-specific. It allows detecting VHH's from multiple germline sequence families. It is conjugated to HiLyte™ Fluor 488, a green fluorophore with brighter fluorescence and better photostability than fluoresceins such as FITC. The fluorescence of HiLyte™ Fluor 488 can be observed at excitation/emission wavelength of 499 nm/523 nm. The degree of substitution (DOS) of this antibody conjugate is batch dependent and reported on the certificate of analysis.
Besides the conventional IgGs (IgG1, composed of heavy and light chains), llamas and other camelids produce single chain IgGs (IgG2 and IgG3). They are composed of heavy chains only and are lacking the light chains and the CH1 domains. The antigen binding domain of IgG2 and IgG3, called VHH, is the smallest naturally occurring antibody binding domain. VHHs have amino acid sequences which are highly variable. They belong to 5 germline sequence families, and multiple mutations are observed. This anti-VHH rabbit secondary antibody recognizes VHHs from multiple framework sequence families.