Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMPs play important roles in tumor development and invasion, as well in many diseases. The SensoLyte® 390 Generic MMP Assay Kit is optimized to detect the activity of a variety of MMPs, including MMP-1, 2, 7, 8, 9, 13, 14, 15, 16, and 24. This kit can be used for detecting generic MMP activity in biological samples or for high throughput screening of MMP inducers and inhibitors using purified MMPs. This kit provides an Mca/Dnp fluorescence resonance energy transfer (FRET) peptide as a MMP substrate. In the intact FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by MMPs, the fluorescence of Mca is recovered, and can be monitored at excitation/emission wavelengths = 330 nm/390 nm. The assays are performed in a convenient 96-well microplate format.
Component A: Mca/Dnp, MMP substrate, Ex/Em=330 nm /390 nm upon cleavage: 50 ml
Component B: Mca fluorescence reference standard, Ex/Em=330nm /390 nm: 1 mM, 10 mL
Component C: APMA, 4-aminophenylmercuric acetate: 1M, 20 mL
Component D: Assay buffer: 20 mL
Component E: Stop Solution: 10 mL
Storage & stability
Store all components at -20°C. Protect Components A and B from light. Components D and E can be stored at 4°C for convenience.