Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. The cathepsin family consists of 12 cysteine proteases with broad exo- and endopeptidase activity, of which Cathepsin B is a member. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD). The SensoLyte® 440 Cathepsin B Assay Kit provides a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin B cleavage and can be detected with excitation at 354 nm and emission at 442 nm.
Component A: Cathepsin B substrate, Ex/Em=354 nm/442 nm upon cleavage: 1 mM, 50 µL
Component B: AMC, Fluorescence Reference Standard, Ex/Em=354 nm/442 nm: 1 mM, 10 µL
Component C: Cathepsin B enzyme, human liver: 5 µL
Component D: Assay Buffer: 20 mL
Component E: Cathepsin B inhibitor Ac-LVK-CHO: 100 µM, 10 µL
Component F: DTT: 1 M, 100 µL
Storage & stability
Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.