Assay-kits

SensoLyte® 440 Cathepsin B Assay Kit Fluorimetric - 1 kit

405,00
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  • Cat.Number : AS-72165
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. The cathepsin family consists of 12 cysteine proteases with broad exo- and endopeptidase activity, of which Cathepsin B is a member. Elevated levels of cathepsin B were detected in metastases and neurological disorders including Alzheimer's disease (AD).
The SensoLyte® 440 Cathepsin B Assay Kit provides a fluorogenic peptide for measurement of enzyme activity. This peptide releases the AMC (7-amino-4-methylcoumarin) fluorophore upon cathepsin B cleavage and can be detected with excitation at 354 nm and emission at 442 nm.

Specifications

Packaging
Kits components
  • Component A: Cathepsin B substrate, Ex/Em=354 nm/442 nm upon cleavage: 1 mM, 50 µL Component B: AMC, Fluorescence Reference Standard, Ex/Em=354 nm/442 nm: 1 mM, 10 µL Component C: Cathepsin B enzyme, human liver: 5 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin B inhibitor Ac-LVK-CHO: 100 µM, 10 µL Component F: DTT: 1 M, 100 µL
Chemistry
UniProt number
  • P07858
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins

Acta Biomater . 2001 Apr 08 ; 10(7) 3288 | DOI : 10.1016/j.actbio.2014.03.036

  • C.M. Vidal
  • et al