Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-13 (collagenase-3, rat collagenase) is involved in quite a few diseases such as arthritis and asthma. The SensoLyte® 490 MMP-13 Assay Kit is a complete assay system designed to continuously analyze MMP-13 activities or to screen MMP-13 inhibitors by using a fluorogenic MMP-13 substrate. Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-13 activity, please choose the SensoLyte® Plus MMP-13 Assay Kit, Cat# AS-72019.
Component A: MMP-13 substrate EDANS/DabcylPlus™ FRET peptide, Ex/Em=340 nm/490 nm upon cleavage: 270 µL
Component B: EDANS, fluorescence reference standard, Ex/Em=340 nm/490 nm: 1 mM DMSO solution, 10 µL
Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL
Component D: Assay buffer: 60 mL
Component E: Stop solution: 30 mL
Storage & stability
Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.