The SensoLyte® Plus 520 MMP-13 Assay Kit is designed for specifically detecting MMP-13 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-13 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-13-induced cleavage of the FRET substrate.
Component A: Microplate coated with monoclonal anti human MMP-13 antibody: 12 x 8 black strips
Component B: MMP-13 standard, recombinant human pro-MMP-13: 10 µg/mL, 10 µL
Component C: MMP dilution buffer: 10 mL
Component D: 10X Wash buffer: 50 mL
Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL
Component F: MMP-13 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL
Component G: Assay buffer: 50 mL
Component H: Stop Solution: 10 mL
Component I: Adhesive cover strip: 3 sheets
Storage & stability
Store all kit components, except Component B, at -20°C. Store Component B at -80°C. For convenience, Components D, G, H, I can be stored at RT.