Assay-kits

SensoLyte® Plus 520 MMP-13 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

555,00
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  • Cat.Number : AS-72019
  • Availability :
    In stock
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The SensoLyte® Plus 520 MMP-13 Assay Kit is designed for specifically detecting MMP-13 activity in biological samples which may contain multiple MMPs, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate. A specific anti-MMP-13 monoclonal antibody is used in combination with a MMP fluorogenic substrate, 5-FAM/QXL™520 FRET peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-13-induced cleavage of the FRET substrate.

Specifications

Packaging
Kits components
  • Component A: Microplate coated with monoclonal anti human MMP-13 antibody: 12 x 8 black strips Component B: MMP-13 standard, recombinant human pro-MMP-13: 10 µg/mL, 10 µL Component C: MMP dilution buffer: 10 mL Component D: 10X Wash buffer: 50 mL Component E: APMA, 4-aminophenylmercuric acetate (Caution: Contain organic mercury. Dispose it according to your local regulations): 100 mM, 150 µL Component F: MMP-13 substrate 5-FAM/QXL™520 FRET peptide Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component G: Assay buffer: 50 mL Component H: Stop Solution: 10 mL Component I: Adhesive cover strip: 3 sheets
Chemistry
UniProt number
  • P45452
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components, except Component B, at -20°C. Store Component B at -80°C. For convenience, Components D, G, H, I can be stored at RT.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 3 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

A novel role of MMP-13 for murine DC function: its inhibition dampens T-cell activation

Int Immunol . 2016 Oct 01 ; 28 473 | DOI : 10.1093/intimm/dxw008

  • J. Bartmann
  • et al

Activation of matrix metalloproteinases 2, 9, and 13 by activated protein C in human osteoarthritic cartilage chondrocytes

Arthritis Rheumatol . 2014 Jun 01 ; 66 1525 | DOI : 10.1002/art.38401

  • M.T. Jackson
  • et al

IL-1β-induced matrix metalloproteinase-13 is activated by a disintegrin and metalloprotease-28-regulated proliferation of human osteoblast-like cells

Exp Cell Res . 2014 Apr 15 ; 323 165 | DOI : 10.1016/j.yexcr.2014.02.018

  • N. Ozeki
  • et al

AP-1 as a regulator of MMP-13 in the stromal cell of giant cell tumor of bone

Biochem Res Int . 2011 Feb 17 ; 2011 164197 | DOI : 10.1155/2011/164197

  • I. Mak
  • et al

Collagenase expression and activity in the stromal cells from giant cell tumour of bone

Bone . 2009 Feb 12 ; 44 865 | DOI : 10.1016/j.bone.2009.01.393

  • R.W. Cowan
  • et al

Proteolytic activity in wound fluids and tissues derived from chronic venous leg ulcers

Wound Repair Regen.  . 2009 Nov 01 ; 17 832 | DOI : 10.1111/j.1524-475X.2009.00547.x

  • A.N. Moor
  • et al

The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities

Biomaterials . 2008 May 01 ; 29 2370 | DOI : 10.1016/j.biomaterials.2008.01.035

  • C. Salinas
  • et al

A role for CITED2, a CBP/p300 interacting protein, in colon cancer cell invasion

FEBS Lett . 2007 Dec 03 ; 581 5904 | DOI : 10.1016/j.febslet.2007.11.072

  • L. Bai
  • et al