Assay-kits

SensoLyte® 520 Generic MMP Activity Kit Fluorimetric - 1 kit

422,00
Check your price
  • Cat.Number : AS-71158
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Select specification

Quantity

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known.
The SensoLyte® 520 Generic MMP Assay Kit is optimized to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14. Compared to the SensoLyte® 520 kits designed for individual MMP, the Generic MMP Assay kit is 5 to 20 fold more sensitive, although less specific. This kit is ideal for detecting generic MMP activity in biological samples or for high throughput screening of MMPs’ inducers and inhibitors using purified MMPs. This kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by SensoLyte®. Upon the cleavage of the FRET peptide by MMPs, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Specifications

Packaging
Kits components
  • Component A: Generic MMP substrate Ex/Em=490 nm/520 nm upon cleavage: 60 mL Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard Ex/Em=490 nm/520 nm: 1 mM, 10 mL Component C: APMA, 4-aminophenylmercuric acetate: 1 M, 20 mL Component D: Assay buffer: 20 mL Component E: Stop solution: 10 mL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect Components A and B from light. Components D and E can be stored at 4°C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • subnanogram
Research Area
Sub-category Research Area
Usage
  • Research use

You may also be interested in the following product(s)

Tube of ADAMTS-4 FRET substrate peptide

ADAMTS-4/Aggrecanase FRET Substrate, WAAG-3R - 1 mg

Cat.Number : AS-60431-1
223,00
Tube of Human MMP-1 (Catalytic Domain), Recombinant protein

MMP-1 (Catalytic Domain), human recombinant

Cat.Number : AS-55575-1
86,00
Image of a kit SensoLyte 520 MMP-9 Assay Kit Fluorimetric

SensoLyte® 520 MMP-9 Assay Kit Fluorimetric - 1 kit

Cat.Number : AS-71155
422,00

Citations

Interleukin-20 receptor expression in the trabecular meshwork and its implication in glaucoma

J Ocul Pharmacol Th . 2014 Jan 23 ; 30(2-3) 267-79 | DOI : 10.1089/jop.2013.0187

  • K.E. Keller
  • et al

Anti-scarring properties of different tryptophan derivatives

PloS one . 2014 Mar 17 ; 9 e91955 | DOI : https://doi.org/10.1371/journal.pone.0091955

  • M.S. Poormasjedi-Meibod
  • et al

Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins

Acta Biomater . 2014 Apr 08 ; 10 3288 | DOI : 10.1016/j.actbio.2014.03.036

  • C.M. Vidal
  • et al

T cell factor 4 is a pro-catabolic and apoptotic factor in human articular chondrocytes by potentiating nuclear factor κB signaling

J Biol Chem . 2013 Apr 19 ; 288 17552 | DOI : 10.1074/jbc.M113.453985

  • B. Ma
  • et al

Mast cell–restricted, tetramer-forming tryptases induce aggrecanolysis in articular cartilage by activating matrix metalloproteinase-3 and -13 zymogens

J Immunol . 2013 Aug 01 ; 191(3) 1404 | DOI : https://doi.org/10.4049/jimmunol.1300856

  • N.J. Margarinos
  • et al

Proof of Concept: Matrix metalloproteinase inhibitor decreases inflammation and improves muscle insulin sensitivity in people with type 2 diabetes

J Inflamm . 2012 Oct 01 ; 9 35 | DOI : 10.1186/1476-9255-9-35

  • K. Frankwich
  • et al

A dynamic model of calcific nodule destabilization in response to monocyte- and oxidized lipid-induced matrix metalloproteinases

Am J Physiol Cell Physiol . 2012 Feb 15 ; 302(4) C658-65 | DOI : 10.1152/ajpcell.00313.2011

  • R. Li
  • et al

A Wnt/ß-catenin negative feedback loop inhibits IL-1-induced MMP expression in human articular chondrocytes

Arthritis Rheumatism . 2012 Aug 01 ; 64(8) 2589-600 | DOI : 10.1002/art.34425

  • M. Ma
  • et al

Angiogenic properties of myofibroblasts isolated from normal human skin wounds

Angiogenesis . 2012 Feb 18 ; 15(2) 199-212 | DOI : 10.1007/s10456-012-9253-5

  • D. Mayrand
  • et al

Repetitive hyperthermia attenuates progression of left ventricular hypertrophy and increases telomerase activity in hypertensive rats

Am J Physiol Heart Circ Physiol . 2012 Mar 16 ; 302(10) H2092-101 | DOI : 10.1152/ajpheart.00225.2011

  • J.I. Oyama
  • et al

Inhibition of matrix metalloproteinases prevents the potentiation of nondeprived-eye responses after monocular deprivation in juvenile rats

Cereb Cortex . 2011 Jun 17 ; 22(3) 725-34 | DOI : 10.1093/cercor/bhr158

  • M. Spolidoro
  • et al

Decreased secretion of MMP by non-lesional late-stage scleroderma fibroblasts after selection via activation of the apoptotic fas-pathway

J Cell Physiol . 2011 Jul 01 ; 226 1907 | DOI : 10.1002/jcp.22520

  • S. Chabaud
  • et al

Deficiency in TIMP-3 incresases cardiac rupture and mortality post-myocardial infarction via EGFR signaling beneficial effects of cetuximab

Basic Res Cardiol . 2011 Jan 18 ; 106(3) 459-71 | DOI : 10.1007/s00395-010-0147-7

  • L. Hammoud
  • et al

Distinct actions of ETA selective versus combined ETA/ETB receptor antagonists in early diabetic kidney disease

J Pharmacol Exp Ther . 2011 Jan 04 ; 338(1) 263-70 | DOI : 10.1124/jpet.111.178988

  • M.A. Saleh
  • et al

Chronic cigarette smoke exposure generates pathogenic T cells capable of driving COPD-like disease in Rag2-/- mice

Am J Respir Critical Care Med . 2010 Feb 04 ; 181(11) 1223-33 | DOI : 10.1164/rccm.200910-1485OC

  • G. Motz
  • et al

Molecular imaging of matrix metalloproteinase activation to predict murine aneurysm expansion in vivo

J Nucl Med . 2010 Jun 16 ; 51(7) 1107-15 | DOI : 10.2967/jnumed.110.075259

  • M. Razavian
  • et al

The fibrotic phenotype of systemic sclerosis fibroblasts varies with disease duration and severity of skin involvement: reconstitution of skin fibrosis development using a tissue engineering approach

J Pathol . 2009 Mar 01 ; 217 534 | DOI : 10.1002/path.2482

  • M.P. Corriveau
  • et al

Tissue inhibitor of metalloproteinase-3 inhibits neonatal mouse cardiomyocyte proliferation via EGFR/JNK/SP-1 signaling

Am J Physiol Cell Physiol . 2009 Feb 11 ; 296(4) C735-45 | DOI : 10.1152/ajpcell.00246.2008

  • L. Hammoud
  • et al

Immune-induced epithelial to mesenchymal transition in vivo generates breast cancer stem cells

Cancer Res . 2009 Mar 10 ; 69(7) 2887-95 | DOI : 10.1158/0008-5472.CAN-08-3343

  • M. Santisteban
  • et al

Doxycycline attenuates isoproterenol- and transverse aortic banding-induced cardiac hypertrophy in mice

J Pharmacol Exp Ther . 2007 Dec 18 ; 324(3) 1196 | DOI : 10.1124/jpet.107.133975

  • M. Errami
  • et al

A Fra-1 dependent, matrix metalloproteinase driven EGFR activation promotes human lung epithelial cell motility and invasion

J Cell Physiol . 2008 Jan 08 ; 216(2) 405-12 | DOI : 10.1002/jcp.21410

  • P. Adiseshaiah
  • et al

A novel formulation of inhaled doxycycline reduces allergen-induced inflammation, hyperresponsiveness and remodeling by matrix metalloproteinases and cytokines modulation in a mouse model of asthma

Biochem Pharmacol . 2007 Jan 09 ; 75(2) 514-26 | DOI : 10.1016/j.bcp.2007.09.012

  • M.M. Gueders
  • et al

Expression of coronin-3 (coronin-1C) in diffuse gliomas is related to malignancy

J Pathol . 2008 Mar 01 ; 214(4) 415-24 | DOI : 10.1002/path.2308

  • D.R. Thal
  • et al