Assay-kits

SensoLyte® 520 Granzyme A Activity Assay Kit, Fluorimetric - 1 kit

422,00
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  • Cat.Number : AS-72260
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Granzymes are a family of structurally related serine proteases. They are stored within the cytotoxic granules of cytotoxic lymphocytes. Granzymes are known to induce target-cell apoptosis. They have the faculty to initiate cell death once entered the target cells (viral or tumor cells). Granzyme A and B are the most abundantly studied.
Granzyme A is the most abundant serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A enters pathogen-containing and tumor cells by the perforin pathway and induces caspase-independent apoptosis. Thus, cells that are resistant to the caspases or Granzyme B (a chief apoptotic mediator) might be sensitive to Granzyme A. Recent studies showed that Granzyme A activates a novel programmed cell death pathway that begins in the mitochondrion. Granzyme A also targets several important nuclear proteins for degradation including histones and DNA damage repair proteins. Granzyme A has proinflammatory activity inducing certain cytokines secretion.

SensoLyte®520 Granzyme A Assay Kit provides a convenient assay for screening of enzyme inhibitors or for detecting the activity of enzyme using a novel internally quenched 5-FAM/QXL® FRET substrate. Upon cleavage by granzyme A, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. This kit can be used to detect the activity of Granzyme A enzyme in biological samples and purified enzyme preparations.

Specifications

Packaging
Kits components
  • • Component A : 5-FAM/QXL®-520 granzyme A substrate, Ex/Em=490/520 nm upon cleavage 2.0 mM, 50 µL • Component B : 5-FAM, fluorescence reference standard, Ex/Em=490/520 nm 2.0 mM, 10 µL • Component C : 2X Assay Buffer 20 mL Component D Inhibitor 10 mM, 10 µL • Component D : Inhibitor 10 mM, 10 µL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • -20°C
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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