Assay-kits

SensoLyte® 520 Granzyme B Activity Assay Kit, Fluorimetric - 1 kit

485,00
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  • Cat.Number : AS-72261
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Granzymes are a family of structurally related serine proteases. They are stored within the cytotoxic granules of cytotoxic lymphocytes. Granzymes are known to induce target-cell apoptosis. They have the faculty to initiate cell death once entered the target cells (viral or tumor cells). Granzyme A and B are the most abundantly studied.
Granzyme B is a serine protease mostly secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It is one of the major mediators of caspase-dependent apoptosis. Recent studies showed that Granzyme B can also be secreted by various non-cytotoxic cells and involved in degradation of extracellular matrix proteins thereby inducing inflammation and impairing wound healing. Thus, if intracellular activity of Granzyme B is mainly related to apoptosis, its extracellular activity has been linked to roles in arthritis, vascular pathologies, and other diseases.

The SensoLyte® 520 Granzyme B Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of Granzyme B activity using a novel internally quenched 5-FAM/QXL®FRET substrate. Upon cleavage by Granzyme B, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The kit can be used to detect the activity of Granzyme B enzyme in biological samples and purified enzyme preparations. This assay can detect as low as 0.1 ng/mL active granzyme B.

Specifications

Packaging
Kits components
  • • Component A : 5-FAM/QXL®-520 granzyme B substrate, Ex/Em=490/520 nm upon cleavage 0.4 mM, 50 µL • Component B : 5-FAM, fluorescence reference standard, Ex/Em=490/520 nm 0.4 mM, 10 µL • Component C : Recombinant human granzyme B 50 µg/mL, 10 µL • Component D : 2X Assay Buffer 20 mL • Component E : Inhibitor 5 mM, 10 µL
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • -20°C
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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