Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. They are involved in diseases such as cancer, arthritis, asthma, angiogenesis, alopecia and metastasis. They are also identified as drug-screening targets. SensoLyte® 520 MMP Substrate Sampler Kit contains a series of FRET peptide substrates for use as fluorogenic indicators to assay MMP protease activities. It provides the best solution for identifying a suitable fluorogenic substrate for a particular MMP assay. All the MMP substrates in the kit are also available in bulk packages.
Component A: 16 different MMP substrates. Ex/Em=490 nm/520 nm upon cleavage: 100 µM, 150 µL each
Component B: 5-FAM-Pro-Leu-OH, fluorescence reference standard Ex/Em=490 nm/520 nm: 1 mM, 10 µL
Component C: APMA, 4-aminophenylmercuric acetate: 1 M, 100 µL
Component D: Assay buffer: 50 mL
Component E: Stop solution: 30 mL
Storage & stability
Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.