Assay-kits

SensoLyte® AFC Caspase Profiling Kit Fluorimetric - 1 kit

644,00
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  • Cat.Number : AS-71116
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage. They are widely used to monitor caspase activity.
The SensoLyte® Caspase Profiling Kit contains a series of AFC-based peptide substrates (Ex/Em=380 nm/500 nm) as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AFC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors.

Specifications

Packaging
Kits components
  • Component A: Caspase microplate: pre-coated with 8 different caspase substrates Ex/Em=380 nm/500 nm upon cleavage: 2 96-well plates (8 x 12 strips) Component B: AFC, fluorescence reference standard Ex/Em=380 nm/500 nm: 10 mM, 20 µL Component C: Assay buffer: 40 mL Component D: DTT: 1 M, 1 mL Component E: 10X Lysis Buffer: 20 mL
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store all components at -20°C. Keep Components A and B away from light
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor.

J Immunol . 2013 Oct 25 ; 191(11) 5702 | DOI : 10.4049/jimmunol.1301604

  • M.K. Jaiswal
  • et al

Endoplasmic reticulum stress induces a caspase-dependent N-terminal cleavage of RBX1 protein in B cells.

J Biol Chem . 2012 Jul 20 ; 387(37) 31223 | DOI : 10.1074/jbc.M111.314583

  • S. Shteingart
  • et al

Zip6-attenuation promotes epithelial-to-mesenchymal transition in ductal breast tumor (T47D) cells.

Exp Cell Res . 2009 Oct 21 ; 316(3) 366 | DOI : 10.1016/j.yexcr.2009.10.011

  • V. Lopez
  • et al