Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage. They are widely used to monitor caspase activity. The SensoLyte® Caspase Profiling Kit contains a series of AFC-based peptide substrates (Ex/Em=380 nm/500 nm) as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AFC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors.
Component A: Caspase microplate: pre-coated with 8 different caspase substrates Ex/Em=380 nm/500 nm upon cleavage: 2 96-well plates (8 x 12 strips)
Component B: AFC, fluorescence reference standard Ex/Em=380 nm/500 nm: 10 mM, 20 µL
Component C: Assay buffer: 40 mL
Component D: DTT: 1 M, 1 mL
Component E: 10X Lysis Buffer: 20 mL
Storage & stability
Store all components at -20°C. Keep Components A and B away from light