Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=380 nm/500 nm). They are widely used to monitor caspase activity. The SensoLyte® Caspase Substrate Kit contains a series of AFC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AFC-based caspase assays. All the components in the kit are available in bulk package.
Component A: Caspase substrates Peptide sequence; Ex/Em=380 nm/500 nm upon cleavage: 8 substrates 10 mM, 70 µL
Component B: AFC, fluorescence reference standard Ex/Em=380 nm/500 nm: 10 mM, 20 µL
Component C: Assay buffer: 80 mL
Component D: DTT: 1 M, 1 mL X 2 vials
Component E: 10X Lysis Buffer: 20 mL
Storage & stability
Store all components at -20°C. Keep Components A and B away from light. For convenience, Components C and E can be stored at 4°C.