Assay-kits

SensoLyte® AFC Caspase Substrate Sampler Kit Fluorimetric - 1 kit

520,00
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  • Cat.Number : AS-71117
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AFC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=380 nm/500 nm). They are widely used to monitor caspase activity.
The SensoLyte® Caspase Substrate Kit contains a series of AFC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AFC-based caspase assays. All the components in the kit are available in bulk package.

Specifications

Packaging
Kits components
  • Component A: Caspase substrates Peptide sequence; Ex/Em=380 nm/500 nm upon cleavage: 8 substrates 10 mM, 70 µL Component B: AFC, fluorescence reference standard Ex/Em=380 nm/500 nm: 10 mM, 20 µL Component C: Assay buffer: 80 mL Component D: DTT: 1 M, 1 mL X 2 vials Component E: 10X Lysis Buffer: 20 mL
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store all components at -20°C. Keep Components A and B away from light. For convenience, Components C and E can be stored at 4°C.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Induction of apoptosis pathways in several cell lines following exposure to the marine algal toxin Azaspiracid

Chem Res Toxicol. . 2012 Jul 02 ; 25(7) 1493 | DOI : 10.1021/tx3001785

  • M.J. Twiner
  • et al

The proapoptotic activity of the Interferon-inducible gene IFI16 provides new insights into its etiopathogenetic role in autoimmunity

J Autoimmun. . 2010 May 21 ; 35(2) 114 | DOI : 10.1016/j.jaut.2010.04.001

  • F. Gugliesi
  • et al