Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AMC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=354 nm/442 nm). They are widely used to monitor caspase activity. The SensoLyte® Caspase Substrate Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AMC-based caspase assays. All the components in the kit are available in bulk package.
Component A: 8 AMC-based caspase substrates; Ex/Em=354nm/442 nm upon cleavage: 10 mM DMSO solution, 70 µL each
Component B: AMC, fluorescence reference standard Ex/Em=354nm/442 nm: 10 mM DMSO solution, 20 µL
Component C: Assay Buffer: 100 mL
Component D: DTT: 1 M, 1 mL X 2 vials
Component E: 10X Lysis Buffer: 20 mL
Storage & stability
Store components A, B, and D at -20°C. Components C and E can be stored at 4°C for convenience. Component A and B protect from light and moisture.