Assay-kits

SensoLyte® AMC Caspase Substrate Sampler Kit Fluorimetric - 1 kit

664.00
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  • Cat.Number : AS-71121
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AMC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=354 nm/442 nm). They are widely used to monitor caspase activity.
The SensoLyte® Caspase Substrate Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. It provides the best solution for identifying a suitable substrate for designing AMC-based caspase assays. All the components in the kit are available in bulk package.

Specifications

Packaging
Kits components
  • Component A: 8 AMC-based caspase substrates; Ex/Em=354nm/442 nm upon cleavage: 10 mM DMSO solution, 70 µL each Component B: AMC, fluorescence reference standard Ex/Em=354nm/442 nm: 10 mM DMSO solution, 20 µL Component C: Assay Buffer: 100 mL Component D: DTT: 1 M, 1 mL X 2 vials Component E: 10X Lysis Buffer: 20 mL
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store components A, B, and D at -20°C. Components C and E can be stored at 4°C for convenience. Component A and B protect from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Combined treatment with quercetin and imperatorin as a potent strategy for killing HeLa and Hep-2 cells

Mol Cell Biochem . 2010 Mar 30 ; 392(1-2) 213 | DOI : 10.1007/s11010-014-2032-4

  • D. Bądziul
  • et al

The effect of quercetin and imperatorin on programmed cell death induction in T98G cells in vitro

Pharmacol Rep . 2014 Mar 04 ; 66(2) 292 | DOI : 10.1016/j.pharep.2013.10.003

  • D. Bądziul
  • et al

Apoptosis induction in human glioblastoma multiforme T98G cells upon temozolomide and quercetin treatment

Tumour Biol. . 2013 Apr 12 ; 34(4) 2367 | DOI : 10.1007/s13277-013-0785-0

  • J. Jakubowicz-Gil
  • et al