α-Synuclein is a major component of Lewy bodies in the affected neurons in Parkinson’s disease. This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal with higher sequence divergence. α-Synuclein is predominantly expressed in brain: specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions. Other tissues express α-Synuclein at very low levels. The physiological role of α-Synuclein is not yet well understood. However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis. This kit is optimized to detect human alpha-Synuclein (α-Synuclein) in cell and tissue lysates, and in human body fluids such as cerebrospinal fluid (CSF) and plasma. Wells are pre-coated with anti-α-Synuclein monoclonal antibodies, blocked, and stabilized for long-term storage. The amount of α-Synuclein is quantified using sandwich ELISA: rabbit polyclonal anti-α-Synuclein specific antibodies directly conjugated to horseradish peroxidase (HRP) are used to detect captured α-Synuclein.
Component A: Anti-a-Synuclein: 8-well strip plate (8x12) 12 x 8 well strips
Component B: a-Synuclein Standard, human (1 µg/vial): 3 vials
Component C: 1X Sample Dilution Buffer: 30 ml
Component D: 10X Wash Buffer: 30 ml
Component E: TMB Color Substrate Solution: 10 ml
Component F: Stop Solution: 10 ml
Component G: Detection Antibody Rabbit Polyclonal anti-a-Synuclein IgG-HRP (10 µg/50µl): 50 µl
Component H: Adhesive Plate Covers: 2 pieces
Storage & stability
Store all kit components at 4C for up to 6 months. Protect components E and G from light.