Glucocerebrosidase/GBA (also called acid β-glucosidase, Glucosylceramidase) is a lysosomal enzyme responsible for the breakdown of glucocerebroside releasing glucose and ceramide. Deficiency of this enzyme due to genetic mutations leads to accumulation of glucocerebroside and development of lysosomal storage disease, known as Gaucher disease (GD). Mutations in the glucocerebrosidase (GBA1) gene are also associated with increased risk for Parkinson disease and related disorders. It has been hypothesized that GBA, when not available to clear out proteins like alpha-synuclein, results in the accumulation of the proteins thereby contributing to Parkinson’s disease.
The SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. The GBA substrate provided in the kit releases the red fluorescent dye resorufin upon Glucocerebrosidase cleavage with absorption/emission maxima at 570 nm/610 nm. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds.