Assay-kits

SensoLyte® Anti-a-Synuclein (Rat) ELISA Kit - 1 kit

485,00
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  • Cat.Number : AS-55550-R
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

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α-Synuclein is a major component of Lewy bodies in the affected neurons in Parkinson’s disease. This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal with higher sequence divergence. α-Synuclein is predominantly expressed in brain: specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions. Other tissues express α-Synuclein at very low levels. The physiological role of α-Synuclein is not yet well understood. However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis.
The SensoLyte α-Synuclein Quantitative ELISA Kit (Rat) provides a convenient and quantitative assay for determining α-Synuclein amount in cell and tissue lysate as well as in body fluids. Compared to other anti-α-Synuclein ELISA kits on the market, it takes less time to run this assay. HRP conjugated detection antibody in this kit is added simultaneously with samples and standards during the assay. This eliminates extra incubation and washing steps and makes this kit one-step procedure for α-Synuclein quantification.

Specifications

Packaging
Kits components
  • Component A: Anti-a-Synuclein 8-well strip plate (8x12): 12 x 8 well strips Component B: a-Synuclein Standard, Rat (1 µg/vial): 3 vials Component C: 1X Sample Dilution Buffer: 30 ml Component D: 10X Wash Buffer: 30 ml Component E: TMB Color Substrate Solution: 10 ml Component F: Stop Solution: 10 ml Component G: Detection Antibody Rabbit Polyclonal anti-rat-a-Synuclein IgG-HRP (10 µg/50µl): 50 µl Component H: Adhesive Plate Covers: 2 pieces
Chemistry
UniProt number
  • P37377
Storage & stability
Storage Conditions
  • Store all kit components at 2-4°C for up to 6 months
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 5 pg/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • rat

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