Image of a kit SensoLyte Generic MMP Assay Kit Colorimetric
Assay-kits

SensoLyte® Generic MMP Assay Kit Colorimetric - 1 kit

€461.00
Check your price
  • Cat.Number : AS-72095
  • Availability :
    In stock
  • Shipping conditions : Ice delivery fees must be applied

Alternative choices

Quantity

Add to wishlist

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known.
The SensoLyte® Generic MMP Assay Kit can be used to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14 or for high throughput screening of MMPs’ inducers and inhibitors. The substrate is a thiopeptolide that is cleaved by the MMPs, releasing a sulfhydryl group, which can be detected with a color-developing thiol-reactive agent, 4,4’-dithiodipyridine or Ellman’s Reagent at 412 nm using a microplate reader. The assays are performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: MMP colorimetric substrate: 10 mM, 100 mL Component B: Reference standard: 10 mM, 10 mL Component C: Assay Buffer: 20 mL Component D: MMP inhibitor: 2 mM, 10 mL Component E: Trypsin: 1 mg/mL, 100 mL Component F: Trypsin inhibitor: 10 mg/mL, 100 mL Component G: Stop Solution: 5 mL
Properties
Absorbance (nm)
  • 412
Storage & stability
Storage Conditions
  • Store all components at -20°C. Store component C at 4°C and protect from the light. Component G can be at room temperature for convenience. Aliquot Components E and F as needed to avoid freeze-thaw cycles.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • nanogram
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

Downloads

You may also be interested in the following product(s)

Tube of ADAMTS-4 FRET substrate peptide

ADAMTS-4/Aggrecanase FRET Substrate, WAAG-3R - 1 mg

Cat.Number : AS-60431-1
€296.00 Excl. Tax
Tube of Human MMP-1 (Catalytic Domain), Recombinant protein

MMP-1 (Catalytic Domain), human recombinant

Cat.Number : AS-55575-1
€119.00 Excl. Tax
Image of a kit SensoLyte 520 MMP-9 Assay Kit Fluorimetric

SensoLyte® 520 MMP-9 Assay Kit Fluorimetric - 1 kit

Cat.Number : AS-71155
€554.00 Excl. Tax

Citations

Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro JDR 91, 192-196

J Dent Res . 2012 Feb 01 ; 91 192 | DOI : 10.1177/0022034511427705

  • A. Tezvergil-Mutluay
  • et al

Inhibition of endogenous dentin matrix metalloproteinases by ethylenediaminetetraacetic acid

J Endod . 2011 Nov 03 ; 38 62 | DOI : 10.1016/j.joen.2011.09.005

  • J. Thompson
  • et al

The Inhibitory Effects of Quaternary Ammonium Methacrylates on Soluble and Matrix-bound MMPs

J Dent Res . 2011 Apr 01 ; 90 535 | DOI : 10.1177/0022034510389472

  • A. Tezvergil-Mutluay
  • et al

The inhibitory effect of polyvinylphosphonic acid on functional MMP activities in human demineralized dentin

Acta Biomater . 2010 May 23 ; 6 4136 | DOI : 10.1016/j.actbio.2010.05.017

  • A. Tezvergil-Mutluay
  • et al