Assay-kits

SensoLyte® Luminescent Secreted Alkaline Phosphatase Reporter Gene Assay Kit Luminometric - 1 kit

362.00
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  • Cat.Number : AS-72145
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The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals. SEAP can be effectively secreted into culture medium and serum. This unique characteristic of SEAP makes it convenient for kinetic analysis of gene expression over a period of time. SensoLyte® Luminescent Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a highly sensitive assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. The kit contains: Chemiluminescent substrate, Assay buffer, Stop solution, Placental alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.

Specifications

Packaging
Kits components
  • Component A: Chemiluminescent substrate: 25 mL Component B: 10X Assay buffer: 50 mL Component C: Human Placental Alkaline Phosphatase Standard: 10 µg/mL, 50 µL Component D: Triton X-100: 500 µL Component E: Stop Solution: 30 mL
Storage & stability
Storage Conditions
  • Store components B and C at -20°C. Store all other components at 4°C. Protect component A from light.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Identification of exosite residues of Factor Xa involved in recognition of PAR-2 on endothelial cells

Biochemistry . 2012 Mar 15 ; 51(12) 2551 | DOI : 10.1021/bi300200p

  • C. Manithody
  • et al