SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit Colorimetric - 1 kit

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  • Cat.Number : AS-72144
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The secreted alkaline phosphatase (SEAP) is widely used as reporter gene to analyze gene expression, the activity of promoters and transcriptional factors in cell culture or animals. SEAP can be effectively secreted into culture medium and serum. This unique characteristic of SEAP makes it convenient for kinetic analysis of gene expression over a period of time. SensoLyte® pNPP Secreted Alkaline Phosphatase Reporter Gene Assay Kit provides a sensitive colorimetric assay of placental alkaline phosphatase for both secreted and membrane-bound form of enzyme. The kit contains: pNPP chromogenic liquid substrate (absorption maximum = 405 nm upon dephosphorylation), Assay buffer, Stop solution, Placental alkaline phosphatase standard, “mix and read” assay protocol that is compatible with high throughput screening.


Kits components
  • Component A: pNPP, colorimetric placental alkaline phosphatase substrate: 25 mL Component B: 10X Assay buffer: 50 mL Component C: Stop solution: 25 mL Component D: Triton-X-100: 500 µL Component E: Human Placental Alkaline Phosphatase Standard: 10 µg/mL, 100 µL
Absorbance (nm)
  • 405
Storage & stability
Storage Conditions
  • Store components B and E at -20°C. All other components at 4°C. Protect component A from light.
Biomarker Target
Detection Method
Detection Limit
  • 1 ng/ml
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

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