Protein phosphatases have received great attention as drug-screening targets. MFP is a proprietary fluorogenic substrate for most phosphatases, i.e., alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases, and serine/threonine phosphatases. The phosphatase-induced hydrolysis of MFP yields an intense fluorescent product that has excitation wavelength around 470 nm, and maximum emission around 510 nm. This characteristic makes the substrate adaptable to all the fluorescence instruments. SensoLyte® MFP Protein Phosphatase Assay Kit uses MFP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. The kit contains: - MFP phosphatase substrate - Protein phosphatase inhibitor - Assay buffer - A 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.
Component A: MFP (1 vial), Ex/Em= 470 nm/510 nm upon phosphate group removal 1 mL
Component B: Assay buffer: 60 mL
Component C: 10X Lysis buffer: 50 mL :
Component D: Triton X-100: 500 µL
Component E: Stop solution: 30 mL
Component F: 1 M DTT: 100 µl
Storage & stability
Store components A and F at -20°C. Component B, C, D, and E can be stored at room temperature for convenience.