SensoLyte® Red Transglutaminase Assay Kit Fluorimetric - 1 kit

Check your price
  • Cat.Number : AS-72245
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

Alternative choices


Transglutaminases (TGs) are Ca2+ and thiol-dependent enzymes that catalyze posttranslational modifications of proteins. These covalent reactions include formation of isopeptide linkages between the carboxamide groups of protein-bound glutamine residues and ε-amino groups of protein-bound lysine and polyamine residues, hydrolysis and esterification of glutamine residues.1 Family of TGs includes at least nine members: TG 1-7, coagulation factor XIIIa, and the keratinocyte membrane bound TG form. TG has attracted considerable interest as a potential drug target. Its activity is thought to contribute to Alzheimer disease, Parkinson disease, Huntington disease, and supranuclear palsy.
SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. TG catalyzes covalent bond formation between a free amine group of poly-D-lysine, which is coated on the plate surface, and γ-carboxamide group of glutamine in biotin-TVQQEL peptide substrate. The reaction immobilizes biotin-conjugated peptide to the plate surface. The amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP), 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), and hydrogen peroxide (H2O2). The non-fluorescent ADHP is oxidized to the strong fluorescent resorufin in the presence of H2O2 by peroxidases, such as HRP. The fluorescence intensity of resorufin is proportional to the activity of TG in a sample. Assay is performed in a convenient 96-well microplate format.


Kits components
  • Component A: 96 well plate, coated with Poly-D-Lysine, includes adhesive sealing film: 1 plate Component B: Wash/Assay buffer (10X): 25 mL Component C: Biotin-peptide substrate: 50 µL Component D: Human transglutaminase-2, recombinant: 2.5µg, 40 µL Component E: Transglutaminase reaction buffer: 15 mL Component F: DTT, 1M: 150 µL Component G: Streptavidin HRP conjugate: 25 µL Component H: ADHP solution: 50 µL Component I: Hydrogen peroxide solution: 50 µL Component J: Transglutaminase inhibitor: 1mM, 25 µL
UniProt number
  • P21980
Absorbance (nm)
  • 545
Emission (nm)
  • 590
Storage & stability
Storage Conditions
  • Store component D at -80°C, components C, F, G, J at -20°C and components A, B, E, H, I at 4°C. Protect components G, H, I from light.
Biomarker Target
Detection Method
Detection Limit
  • 120 ng/ml
Research Area
Sub-category Research Area
  • Research use
Code Nacres
  • NA.32

You may also be interested in the following product(s)

Tube of EndoClearplus alpha–synuclein, Human Recombinant protein

EndoClear™ Plus human recombinant a-Synuclein protein

Cat.Number : AS-56081-100
263.00 Excl. Tax
Image of a kit SensoLyte 520 BACE2 Activity Assay Kit Fluorimetric

SensoLyte® 520 BACE2 Activity Assay Kit Fluorimetric - 1 kit

Cat.Number : AS-72225
554.00 Excl. Tax