Assay-kits

SensoLyte® Rh110 Cathepsin K Assay Kit Fluorimetric - 1 kit

462,00
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  • Cat.Number : AS-72152
  • Availability :
    In stock
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Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis.
The SensoLyte® Rh110 Cathepsin K Assay Kit provides a convenient assay for high throughput screening of enzyme inhibitors or for continuous assay of enzyme activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496/520 nm.

Specifications

Packaging
Kits components
  • Component A: Rh110 Cathepsin K substrate, Ex/Em=490 /520 nm upon cleavage: 1.4 mM, 50 µL Component B: Rh110, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1.4 mM, 20 µL Component C: Procathepsin K, human recombinant: 0.15 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin K Activation Buffer: 100 µL Component F: Cathepsin K Inhibitor: 100 µM, 10 µL Component G: DTT: 1 M, 200 µL
Chemistry
UniProt number
  • P43235
Properties
Absorbance (nm)
  • 496
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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