Assay-kits

SensoLyte® Transglutaminase Activity Assay Kit Colorimetric - 1 kit

422,00
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  • Cat.Number : AS-72244
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    In stock
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Transglutaminases (TGs) are Ca2+ and thiol-dependent enzymes that catalyze posttranslational modifications of proteins. These covalent reactions include formation of isopeptide linkages between the carboxamide groups of protein-bound glutamine residues and ε-amino groups of protein-bound lysine and polyamine residues, hydrolysis and esterification of glutamine residues. Family of TGs includes at least nine members: TG 1-7, coagulation factor XIIIa, and the keratinocyte membrane bound TG form. TG has attracted considerable interest as a potential drug target. Its activity is thought to contribute to Alzheimer disease, Parkinson disease, Huntington disease, and supranuclear palsy.
SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. TG catalyzes covalent bond formation between a free amine group of poly-D-lysine, which is coated on the plate surface, and ?-carboxamide group of glutamine in biotin-TVQQEL peptide substrate. The reaction immobilizes biotin-conjugated peptide to the plate surface. The amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP) and TMB substrate. Colorimetric signal generated by HRP and TMB substrate is proportional to the activity of TG in a sample. Assay is performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: 96 well plate, coated with Poly-D-Lysine, includes adhesive sealing film: 1 plate Component B: Wash/Assay buffer (10X): 25 mL Component C: Biotin-peptide substrate: 50 µL Component D: Human transglutaminase-2, recombinant: 2.5µg, 40 µL Component E: Transglutaminase reaction buffer: 15 mL Component :F DTT, 1M: 150 µL Component G: Streptavidin HRP conjugate: 25 µL Component H: TMB substrate: 10 mL Component I: Stop solutio:n 10 mL Component J: Transglutaminase inhibitor: 1mM, 25 µL
Chemistry
UniProt number
  • P21980
Properties
Absorbance (nm)
  • 450
Storage & stability
Storage Conditions
  • Store component D at -80°C; Store components C, F, G, and J at -20°C; Store components A, B, E, H, and I at 4°C; Protect components G, H from light.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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