Ultra pure Diamond Taq® Benefits
Our IVD enzymes are highly suited for Diagnostic kits, Lab Services (with ASRs) and demanding Research
Stringent process and QC ensuring lot-to-lot & results reproducibility
Quality Management System fully compliant to ISO 13 485 Medical Device standards and FDA’s Quality System Regulations
Cost-effective tailored solution
Ultra-low residual DNA content
<1 fg of genomic E. coli DNA / Taq Unit Typically < 0.01 fg or 0.002 E. coli genome copies /Taq Unit
Highly pure (> 98 %) and ultra-low bioburden (≤ 10 CFU/ml). Typically = 0 CFU/ml
Amplification of DNA templates even at very low concentrations
How to select a polymerase?
|PCR||qPCR||Yield||Sensitivity Low Copy Template||Specificity||Difficult Template (GC & AT rich)||IP Burden||Free Sample|
|Hot Diamond Taq®
Short activation time
|HGS Diamond Taq®
Heat stability improved
Hot Diamond Taq®
The Hot Diamond Taq® represents a completely new “HotStart Concept”. Neither accomplished through chemical modification nor a blocking antibody but a proprietary agent. This polymerase needs a really short activation time (20s are sufficient) and remains compatible with all existing protocols.
Fig: Hot Diamond Taq® was evaluated using from 20 seconds to 10 minutes as activation time for its ability to amplify 1 and 5 pg of λDNA. PCR products were analyzed by gel electrophoresis.
All these enzymes, shipped on dry ice, follow the IVD traceability
at each step of the process, a Tracking number is sent to customer the day of the shipment.
|Identity (SDS-PAGE)||MW approx. 95 kDa|
|Volume activity||≥ 5U/µl|
|Purity (SDS-PAGE)||> 98%|
|Performance test: PCR-λ DNA*||0.5 kb fragment positive down to 5 pg|
|Performance test: PCR – 18S DNA*||0.1 kb fragment positive down to 10 pg|
|Ribonucleases (up to 10U, 1h, 37°C)||Not detectable|
|Endonucleases, Exonucleases, Nicking activity (up to 30U, 16h, 65°C)||Not detectable|
|E.coli residual DNA||< 1 fg/Taq Unit|
|Bioburden*||≤ 10 CFU/ml|
|Stability*||24 months (at –20°C) from date of manufacture|
|HotStart||No detectable amplification without activation|
|Performance test PCR-Numb DNA (Hot Diamond Taq®)||0.3 kb fragment positive down to 10 pg|