Biomarkers are optimal for reading DNA fragments ranging from 50 to 10000 bp. They are stable at room temperature and are supplied dephosphorylated in order to allow labelling with polynucleotide kinase onto free 5’-termini.
Biotin and Rhodamine labelled biomarkers are available.These markers are evaluated for resolution, intensity and background on 7.5 % acrylamide and 3:1 SFAgarose: Agarose gels with ethidium bromide staining.
BioMarker® EXT PLUS contains linear double stranded DNA bands of 50, 100, 200, 300, 400, 500, 525, 700, 1000, 1500, 2000 and 2500 base pairs. The concentration of each band is 50 ng/5 µl of BioMarker® EXT PLUS applied except for 1000 bp which is at 100 ng/5 µl applied.