Assay-kits

SensoLyte® 490 MMP-12 Assay Kit Fluorimetric - 1 kit

611,00 427,70 30.00 %
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  • Cat.Number : AS-71137
  • Availability :
    In stock
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is implicated in the pathology of many diseases such as emphysema, aortic lesions and cancer. It has recently been shown to play a role in the pathogenesis of rheumatoid arthritis. The SensoLyte® 490 MMP-12 Assay Kit is a complete assay system designed to continuously analyze MMP-12 activity or to screen for MMP-12 inhibitors by using a fluorogenic MMP-12 substrate.

Specifications

Packaging
Kits components
  • Component A: MMP-12 substrate EDANS/DabcylPlus™ FRET peptide, Ex/Em=340 nm/490 nm upon cleavage: 270 µL Component B: EDANS, fluorescence reference standard, Ex/Em=340 nm/490 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL Component D: Assay buffer: 60 mL Component E: Stop solution: 30 mL
Chemistry
UniProt number
  • P39900
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect Components A and B from light. Components D and E can be stored at 4°C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 43 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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Citations

Expression of matrix metalloproteinase-12 in aortic dissection

BMC Cardiovasc Disord . 2013 May 03 ; 13 34 | DOI : 10.1186/1471-2261-13-34

  • Y. Song
  • et al

Inhibitory effect of Chinese green tea on cigarette smoke-induced up-regulation of airway neutrophil elastase and matrix metalloproteinase-12 via antioxidant activity

Free Radic Res. . 2012 Jun 08 ; 46(9) 1123 | DOI : 10.3109/10715762.2012.692786

  • K.H. Chan
  • et al

Inflammatory signatures for eosinophilic versus neutrophilic allergic pulmonary inflammation reveal critical regulatory checkpoints

Am J Physiol Lung Cell Mol Physiol. . 2011 Feb 18 ; 300(5) L679 | DOI : 10.1152/ajplung.00202.2010

  • P. Bogaert
  • et al

Matrix metalloproteinase 12 overexpression in lung epithelial cells plays a key role in emphysema to lung bronchioalveolar adenocarcinoma transition

Cancer Res . 2009 Aug 25 ; 69(18) 7252 | DOI : 10.1158/0008-5472.CAN-09-0577

  • P. Qu
  • et al

Upregulation of elastase proteins results in aortic dilatation in mucopolysaccharidosis I mice

Mol Genet Metab . 2008 Mar 13 ; 94(3) 298 | DOI : 10.1016/j.ymgme.2008.03.018

  • X. Ma
  • et al

Alveolar macrophage activation and an emphysema-like phenotype in adiponectin-deficient mice

Am J Physiol Lung Cell Mol Physiol. . 2008 Mar 07 ; 294(6) L1035 | DOI : 10.1152/ajplung.00397.2007

  • R. Summer
  • et al

Elevated MMP-12 protein levels in induced sputum from patients with COPD

Thorax . 2005 Nov 24 ; 61(3) 196 | DOI : 10.1136/thx.2005.042432

  • I.K. Demedts
  • et al