Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is implicated in the pathology of many diseases such as emphysema, aortic lesions and cancer. It has recently been shown to play a role in the pathogenesis of rheumatoid arthritis. The SensoLyte® 490 MMP-12 Assay Kit is a complete assay system designed to continuously analyze MMP-12 activity or to screen for MMP-12 inhibitors by using a fluorogenic MMP-12 substrate.
Component A: MMP-12 substrate EDANS/DabcylPlus™ FRET peptide, Ex/Em=340 nm/490 nm upon cleavage: 270 µL
Component B: EDANS, fluorescence reference standard, Ex/Em=340 nm/490 nm: 1 mM, 10 µL
Component C: APMA, 4-aminophenylmercuric acetate (Caution: Toxic! Handle with care): 1 M, 100 µL
Component D: Assay buffer: 60 mL
Component E: Stop solution: 30 mL
Storage & stability
Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.