Assay-kits

SensoLyte® AMC Caspase Profiling Kit Fluorimetric - 1 kit

644,00
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  • Cat.Number : AS-71120
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AMC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=354 nm/442 nm). They are widely used to monitor caspase activity.
The SensoLyte® Caspase Profiling Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AMC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors.

Specifications

Packaging
Kits components
  • Component A: AMC-based caspase substrates, Ex/Em=354 nm/442 nm upon cleavage Two 96-well plates pre-coated with 8 different caspase substrates. Component B: AMC, fluorescence reference standard Ex/Em=354nm/442 nm: 10 mM DMSO solution, 20 µL Component C: Assay Buffer, add DTT before use: 40 mL Component D: DTT: 1 M, 1 mL Component E: Caspase dilution buffer: 20 mL Component F: 10X Lysis Buffer: 20 mL
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store all kit components at -20°C. Protect Components A and B from light and moisture
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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