Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AMC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=354 nm/442 nm). They are widely used to monitor caspase activity. The SensoLyte® Caspase Profiling Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AMC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors.
Component A: AMC-based caspase substrates, Ex/Em=354 nm/442 nm upon cleavage Two 96-well plates pre-coated with 8 different caspase substrates.
Component B: AMC, fluorescence reference standard Ex/Em=354nm/442 nm: 10 mM DMSO solution, 20 µL
Component C: Assay Buffer, add DTT before use: 40 mL
Component D: DTT: 1 M, 1 mL
Component E: Caspase dilution buffer: 20 mL
Component F: 10X Lysis Buffer: 20 mL
Storage & stability
Store all kit components at -20°C. Protect Components A and B from light and moisture