Assay-kits

SensoLyte® Cell Viability and Proliferation Assay Kit Fluorimetric, Large size - 1 kit

555,00
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  • Cat.Number : AS-71301
  • Availability :
    In stock
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Lactate dehydrogenase (LDH) exists ubiquitously in the cytoplasm of living mammalian cells and the measurement of cytoplasmic LDH activity is a well-accepted assay to quantify living cell numbers and indicate cell viablity. The DHL™ Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is be converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (r2=0.99). This kit can also be used for high throughput screening of cell proliferation or cytotoxicity effect of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 10,000 assays

Specifications

Packaging
Kits components
  • Component A: Assay solution: 20 mL X 10 bottles
Properties
Absorbance (nm)
  • 560
Emission (nm)
  • 590
Storage & stability
Storage Conditions
  • Store the entire kit at -20°C, and keep away from light
Activity
Application
Detection Method
Detection Limit
  • 48-50000 cells
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

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Signaling pathways involved in cyclooxygenase-2 induction by hepatocyte growth factor in non-small cell lung cancer

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