Assay-kits

SensoLyte® Rh110 Cathepsin G Assay Kit Fluorimetric - 1 kit

465,00
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  • Cat.Number : AS-72186
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

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Quantity

Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin G is the serine protease released by neutrophils upon their activation. Cathepsin G is currently being explored as a target for anti-inflammatory and anti-infective drugs.
The SensoLyte® Rh110 Cathepsin G Assay Kit provides a convenient assay for high throughput screening of enzyme inhibitors or for continuous assay of enzyme activity utilizing a fluorogenic peptide. Upon enzyme cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496/520 nm.

Specifications

Packaging
Kits components
  • Component A: Cathepsin G substrate, Ex/Em=490/520 nm upon cleavage: 55 µL Component B: Rh110, fluorescence reference standard, Ex/Em=490 nm/520 nm: 3 mM, 10 µL Component C: Cathepsin G, Human Neutrophil: 0.1 mg/mL, 15 µL Component D: Assay Buffer: 25 mL Component E: Cathepsin G Inhibitor: 100 µM, 20 µL
Chemistry
UniProt number
  • P08311
Properties
Absorbance (nm)
  • 496
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except for Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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