Assay-kits

SensoLyte® Rh110 Cathepsin L Assay Kit Fluorimetric - 1 kit

465.00
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  • Cat.Number : AS-72217
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    In stock
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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins.
The SensoLyte® Rh110 Cathepsin L Activity Assay Kit provides a convenient assay for screening Cathepsin L inhibitors or assay of enzyme activity using a fluorogenic substrate. Upon cleavage by Cathepsin L, this substrate generates the Rh110 (rhodamine 110) fluorophore with a bright green fluorescence that can be detected at excitation/emission=490/520 nm. The longer wavelength spectra and higher extinction coefficient of Rh110 provide greater sensitivity and less interference from other reaction components.

Specifications

Packaging
Kits components
  • Component A: Rh110 Cathepsin L substrate, Ex/Em=490 nm/520 nm upon cleavage: 600 µM, 50 µL Component B: Rh110 fluorescence reference standard, Ex/Em=490 nm/520 nm: 600 µM, 10 µL Component C: Cathepsin L, human recombinant: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin L inhibitor: 100 µM, 10 µL Component F: DTT: 1 M, 200 µL
Chemistry
UniProt number
  • P07711
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all kit components at -20°C, except Component C. Store Component C at -80°C. Protect Components A and B from light and moisture. Component D can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Codes
Code Nacres
  • NA.32

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