Protein

Sortase A protease, recombinant - 10 µg

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  • Cat.Number : AS-72229
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

Quantity

Sortases are a family of membrane–anchored transpeptidases expressed by Gram–positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Since Sortases are widely distributed among a variety of bacterial pathogens and required for virulence, Sortases represent a promising therapeutic target for the development of novel anti-infective agents. In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation.

Sortase A from Staphylcoccus aureus was expressed in E. coli expression system with a N-terminal His tag. It is comprised of 26-206 amino acids and its MW is 21.7-kDa. The activity of Sortase A can be measured in a FRET-based enzymatic assay

Specifications

Chemistry
UniProt number
  • Q2FV99
Molecular Mass/ Weight
  • 21.7 kDa
Modification
Conjugation
  • Unconjugated
Quantity & Purity
Concentration
  • 50 µg/ml
Purity
  • >87%
Storage & stability
Form
  • In solution
Storage Buffer
  • 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, and 2.2 mM KCl, 8 mM imidazole, 0.04% Tween 20, and 20 % glycerol.
Storage Conditions
  • Store at -80 ºC. Avoid repeated freeze-thaw cycles.
Activity
Application
Biomarker Target
Specificity
  • amino acids 26-206 of S. aureus Sortase A
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host
Source / Species
  • Staphylococcus aureus

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