Takyon™ Dry No Rox One-Step RT Probe MasterMix dTTP contains the Takyon™ fusion enzyme for fast, consistent and sensitive results. The Takyon™ reagents are provided lyophilized in bottles of 50 (20 µL) RXN.
Other formulations are available on request
3 bottles of Lyophilized MasterMix (50 RXN) containing Takyon™ DNA polymerase, Euroscript II RT, RNase Inhibitor, dNTPs, MgCl2 (5.5 mM final concentration), Stabilizers; 2 x 1,6 mL Buffer S (Blue cap); 1 x 2 mL PCR grade water (Clear cap)
Resuspend the Takyon™ Dry MasterMix dTTP as per follow: 1) Add the appropriate volume of buffer S or PCR grade water: - 2X : 525 µL PCR grade water or buffer S - 5X : 210 µL PCR grade water or buffer S 2) Close the bottle and stir gently for 15 sec
Dry, Buffer S or PCR grade water
Takyon™ Dry is stable for 18 months at 15-35°C dried. Once resuspended in PCR grade water, use upon resuspension. Once resuspended in Buffer S, it is stable for 24 hours at 4°C or 1 month at -20°C.