Polyclonal antibody fragmentation

Rabbit-lgG's contain only a few sites where pepsin or papain is able to cleave; these antibodies are therefore quite easy to fragment.



Monoclonal antibody fragmentation

The efficiency of the fragmentation reaction is strongly dependent on the subclass of the antibody, because some of these contain multiple cleavage sites.

These mAb subclasses can be classified based on their overall susceptibility to degradation, IgG2b being the most sensitive: lgGl > lgG2e > lgG3 > lgG2b.

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