Each oligonucleotide deserves the right purification method

The aim of any purification method is to remove the by-products resulting from the removal of the protecting groups and other synthesis by products.

When choosing the most appropriate purification method for your oligo you must take into account various parameters such as the oligo length, the presence of modifications and your application.

We offer purifications reaching up to 95% purity level

SePOP desalting increase the purity level of the deprotected and desalted oligos up to 65-70%. It uses differential precipitation to eliminate the largest part of contaminants (truncated material < 10 bases).

RP Cartridge•Gold consists of a reverse phase chromatography based on the difference in hydrophobicity between the full-length product and truncated sequences. It yields to 75-80% purity. It is the best compromise for most applications and the absence of residues (which may occur with HPLC) makes them suitable for cell culture uses.

HPLC provides a degree of purity of at least  85%. Reverse Phase (RP) is based on the hydrophobic interaction of the full-length oligonucleotides with alkyl chains bonded on the matrix. Ion exchange (IEX) is based on the preference of the anion-exchange resins (positively charged) for the full-length oligonucleotides.

In vivo oligonucleotides are the smart choice for antisense oligonucleotides or siRNA testing at a research level before entering into preclinical studies. The production process of in vivo oligonucleotides includes the following steps: IEX-HPLC purification, desalting, filtration and lyophilization.

Polyacrylamide Gel Electrophoresis (PAGE) separates oligonucleotides varying from only 1 base and gives a purity level of 85-90%. Gel band is excised under low intensity UV. Oligonucleotide is then eluted, precipitated, quantified and packaged.

Dual HPLC (double RP or RP+IEX) increases the purity level up to 95%

Applications vs Purifications

Applications (by alphabetical order) Recommended Purification
AFLP RP Cartridge•Gold
Capillary sequencing HPLC
Cloning and subcloning PCR Polyacrylamide Gel Electrophoresis (PAGE)
Cycle sequencing SePOP desalting
DNA MicroArray SePOP desalting
First-strand cDNA synthesis Polyacrylamide Gel Electrophoresis (PAGE)
Gel-shift assay Polyacrylamide Gel Electrophoresis (PAGE)
Gene synthesis Polyacrylamide Gel Electrophoresis (PAGE)
Hybridization SePOP desalting
in situ Hybridization HPLC
in vivo studies In vivo
Isothermal sequencing SePOP desalting
miRNA, siRNA and antisense HPLC
OLA RP Cartridge•Gold
PCR primers RP Cartridge•Gold
Production of cloning linkers Dual HPLC
Real-Time qPCR HPLC
Routine PCR SePOP desalting
Sensitive PCR (Diagnostic) RP Cartridge•Gold
Site-directed mutagenesis Dual HPLC
SNP Analysis SePOP desalting


  • In vivo oligonucleotides

    The ideal choice for your animal studies

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  • Large-scale oligonucleotides

    Get bulk quantities of purified oligonucleotides

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