Endotoxin tested production process

The specific production process has been endotoxin tested. Endotoxins were undetectable using a chromogenic LAL endotoxin assay.

Please note that in vivo purified oligonucleotides are RUO oligos which are not endotoxins free certified.

  1. Ion-Exchange HPLC

    The charge difference between the full-length oligonucleotides and truncated forms is exploited by using anion-exchange chromatography to separate them with high resolution.
  2. Desalting

    Following HPLC residual salts are removed by size-exclusion chromatography. This step is crucial in order to reduce toxicity to living organisms or cells. Osmolarity: < 100 mOsm/Kg.
  3. Filtration

    After desalting oligonucleotides are 0.22 µm filtered. Please note that in vivo purified oligonucleotides are not sterile certified.
  4. Lyophilization

    All in vivo oligonucleotides are lyophilized and shipped dried

Ideal for siRNA-based applications

In vivo oligonucleotides are the smartest choice for antisense oligonucleotides or siRNA testing at a research level before entering into pre-clinical studies.

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Increase Performance
of your oligos

Modified bases such as 2’OMe, LNA or MOE bases are available. Up to 5 Phosphorothioate linkages can be included in order to increase nuclease resistance and stability.
Please note that phosphorothioate linkages are not allowed for siRNA Duplexes.

Solution before entering
Pre-clinical testing

Comparison between the SePOP and HPLC purifications and the in vivo process for a siRNA duplex.

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SePOP Desalted IEX/RP-HPLC In Vivo*
Desalting by precipitation
Desalting by size exclusion chromatography
0.22 µm filtration  

*Production process endotoxin tested