The specific production process has been endotoxin tested. Endotoxins were undetectable using a chromogenic LAL endotoxin assay.
Ion-Exchange HPLCThe charge difference between the full-length oligonucleotides and truncated forms is exploited by using anion-exchange chromatography to separate them with high resolution.
DesaltingFollowing HPLC residual salts are removed by size-exclusion chromatography. This step is crucial in order to reduce toxicity to living organisms or cells. Osmolarity: < 100 mOsm/Kg.
FiltrationAfter desalting oligonucleotides are 0.22 µm filtered. Please note that in vivo purified oligonucleotides are not sterile certified.
LyophilizationAll in vivo oligonucleotides are lyophilized and shipped dried
Solution before entering
Comparison between the SePOP and HPLC purifications and the in vivo process for a siRNA duplex.
|SePOP Desalted||IEX/RP-HPLC||In Vivo*|
|Desalting by precipitation||•||•||•|
|IEX/RP HPLC||•||• (IEX only)|
|Desalting by size exclusion chromatography||•|
|0.22 µm filtration||•|