Tags Oligonucleotides
Pre-diagnostic & Diagnostic Oligonucleotides
@**************************************************************** Header *****************************************************************************@ Thank you for your request Our specialists will take care of your request and contact you soon. } else{ } } @************************************...
Glen Synthesis Reagents
@**************************************************************** Header *****************************************************************************@ Thank you for your request Our specialists will take care of your request and contact you soon. } else{ } } @************************************...
In vivo Oligos
@**************************************************************** HeaderWhite *****************************************************************************@ ? customIlluBg : ""; } Oligos for Clinical Studies We manufacture oligos for pre-clinical and therapeutic applications } @******************...
Access ™ Dyes & Quenchers
@**************************************************************** Header Multi*****************************************************************************@ @********************************************************************************* End OneColumn02 *****************************************...
RNAi
@**************************************************************** HeaderWhite *****************************************************************************@ } else{ } } @************************************************************************** FormStack End **************************************...
Why are my custom oligos delivered at ambient temperature?
Custom oligonucleotides shipped dried or in solution are stable for 2 weeks at room temperature when unopened and protected from light.For long-term storage please check our stability statement.
Meet our experts at "TIDES Europe"
To learn more about our products and services, come and visit us at our booth. For more information, visit the event website.
Oligo Design Assistance
@**************************************************************** HeaderWhite *****************************************************************************@ Thank you for your request Our specialists will take care of your request and contact you soon. } else{ } } @*******************************...
Track Oligonucleotides
@**************************************************************** Header *****************************************************************************@ Thank you for your request Our specialists will take care of your request and contact you soon. } else{ } } @************************************...
Complex Oligonucleotides
@**************************************************************** HeaderWhite *****************************************************************************@ Thank you for your request Our specialists will take care of your request and contact you soon. } else{ } } @*******************************...
ASR Oligonucleotides
@**************************************************************** Header *****************************************************************************@ Thank you for your request Our specialists will take care of your request and contact you soon. } else{ } } @************************************...
Peptide-oligonucleotide conjugates
@**************************************************************** HeaderWhite *****************************************************************************@ Thank you for your request Our specialists will take care of your request and contact you soon. } else{ } } @*******************************...
What cells can be used for RNAi studies?
Most readily transfectable cell lines are suitable for RNAi studies. However, each new assay should be first optimized (i.e. by using a range of siRNA concentrations, by testing different (transfection reagent volume - siRNA amount) ratios ...) F or a successful optimization, pay attention to the...
What kind of negative control can be used ?
Every researcher would tell it: "The choice of the right controls makes the whole difference between a good and a bad experiment". This adage is particularly true for RNAi studies. Therefore, to maximize your result interpretation, the following precautions should be taken when using siRNAs: - Al...
In which form do I receive my siRNA ?
When you order one of our siRNA sets, you receive single-strand siRNAs in separate tubes, either lyophilized (PAGE purified) or desalted. In addition, Eurogentec sends aliquots of Annealing buffer 5X and RNase-free water along with each siRNA oligo set. Such conditioning may seem bothering but it...
What chemistry is used to synthesize Eurogentec's siRNA ?
Although similar to DNA synthesis, the additional 2'-OH group of RNA introduces considerable complexity to the RNA synthesis and requires a different protecting group. All RNA oligos at Eurogentec (including siRNA) are made using monomers containing a 2'-O-tertiary-Butyl-dimethylsylil (TBDMS) pro...
What can I do if the designed siRNA doesn' t work ?
Despite its extreme efficiency, the selected siRNA might not work in your cell system. If so, it is advisable to check the following points: If no knock-out of the target gene is observed, it may be useful to analyze whether the corresponding mRNA was effectively degraded upon addition of the siR...
Why using a 2 pyrimidines overhang at the 3' ends of my siRNA ?
Several studies have shown that the most efficient siRNA contains a dTdT (or UU) overhang at their 3' ends. Using dTdT is recommended for optimal stability of the siRNA duplex, however, UU overhangs work equally well.
How does Eurogentec select target sequences ?
We use bioinformatics tools to select siRNA oligos with characteristics perfectly matching the last experimental results in this field.
Why using synthetic siRNA instead of in vitro transcribed ones ?
In vitro transcription yields RNA molecules that are equally suitable for RNAi studies. Nevertheless, these experiments require expert skills at each step: in vitro transcription, purification, quantification... As a consequence, we frequently meet scientists irritated by the poor quality results...
Why using siRNA instead of other antisense oligonucleotides ?
The discovery of an efficient and easy way to knock out gene expression at the mRNA level has resembled the seek of the Holy Grail for more than 10 years. Unfortunately, the use of classical antisense techniques (i.e. using various chemically modified forms of oligonucleotides) has often resulted...
Can unpurified oligonucleotides be used as PCR primers?
Yes, apparently so. Provided that the PCR primer is not very long (up to 30 bases) and the PCR product is not long, unpurified oligonucleotides will be efficient PCR primers. However, it is difficult to determine the quantity/concentration of a crude PCR primer because it will contain impurities ...
What can I do to my PCR primer to change the mobility of the PCR product?
Addition of multiple thymidine residues at the 5'-end of a PCR primer will change the mobility of the PCR product. Large number of T residues can be added. An alternative is to use a non-coding hydrophilic monomer. The best choice is hexaethylene glycol (hexaethylene oxide). Multiple additions of...